A simplified, orientation-specific cDNA cloning strategy
نویسندگان
چکیده
منابع مشابه
A simplified, orientation-specific cDNA cloning strategy.
D) DIGESTION WITH Hind III AND EcoRI PROTOCOL, a) cDNA synthesis by Reverse Transcriptase is initiated with oligo(dT) according to Gubler and Hofmann (1) . b) The double stranded cDNA is methylated, and (c) a synthetic CCTTGAATTCAAGCNH oligonucleotlde pGCTTGAATTCAAGC CCAACTTAACTTCCNN is added. This generates a Hindlll recognition sequence (AAGCTT) when ligated to the oligo (dA) track at the 3 t...
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Research Objective A researcher used differential cDNA cloning to isolate a cDNA transcripts representing growth factor induced genes. One of these cDNA clones corresponded to a previously uncharacterized open reading frame in the human genome which the exasperated researcher named "Another Moronic Gamble" (AMG). Since increased steady-state mRNA levels could be due to either transcriptional in...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1987
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/15.8.3629